Light Irradiation Induces Fragmentation of the Plasmodium, a Novel Photomorphogenesis in the True Slime Mold Physarum polycephalum: Action Spectra and Evidence for Involvement of the Phytochrome¶

2001 ◽  
Vol 73 (3) ◽  
pp. 324 ◽  
Author(s):  
Yasutaka Kakiuchi ◽  
Tetsuo Takahashi ◽  
Akio Murakami ◽  
Tetsuo Ueda
Keyword(s):  
Physarum Polycephalum ◽  
Slime Mold ◽  
Light Irradiation ◽  
Action Spectra

Ca-Histochemistry in slime mold plasmodia

1978 ◽  
Vol 36 (2) ◽  
pp. 130-131
Author(s):  
Ulrich Dierkes
Keyword(s):  
Physarum Polycephalum ◽  
Carbon Coating ◽  
Freeze Drying ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Slime Mold ◽  
Staining Procedure ◽  
Protoplasmic Streaming ◽  
Intracellular Ca

Calcium is supposed to play an important role in the control of protoplasmic streaming in slime mold plasmodia. The motive force for protoplasmic streaming is generated by the interaction of actin and myosin. This contraction is supposed to be controlled by intracellular Ca-fluxes similar to the triggering system in skeleton muscle. The histochemical localisation of calcium however is problematic because of the possible diffusion artifacts especially in aquous media.To evaluate this problem calcium localisation was studied in small pieces of shock frozen (liquid propane at -189°C) plasmodial strands of Physarum polycephalum, which were further processed with 3 different methods: 1) freeze substitution in ethanol at -75°C, staining in 100% ethanol with 1% uranyl acetate, and embedding in styrene-methacrylate. For comparison the staining procedure was omitted in some preparations. 2)Freeze drying at about -95°C, followed by immersion with 100% ethanol containing 1% uranyl acetate, and embedding. 3) Freeze fracture, carbon coating and SEM investigation at temperatures below -100° C.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

scholarly journals Purification and characterization of a novel intracellular acid proteinase from the plasmodia of a true slime mold, Physarum polycephalum.

1990 ◽  
Vol 265 (32) ◽  
pp. 19898-19903
Author(s):  
K Murakami-Murofushi ◽  
T Takahashi ◽  
Y Minowa ◽  
S Iino ◽  
T Takeuchi ◽  
...  
Keyword(s):  
Physarum Polycephalum ◽  
Slime Mold ◽  
Acid Proteinase ◽  
Purification And Characterization ◽  
Intracellular Acid

Measurement of chemotaxis in the slime mold Physarum polycephalum

1978 ◽  
Vol 116 (2) ◽  
pp. 365-375 ◽  
Author(s):  
Randall L. Kincaid ◽  
Tag E. Mansour
Keyword(s):  
Physarum Polycephalum ◽  
Slime Mold
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